Wen-Yih Chen
National Central University, Taiwan
Title: Investigating Surface Modification of Silicon Nanowire Field-Effect Transistor Biosensors to Quantify Cardiac Troponin I at High Resolution for Early-Stage Diagnosis of Acute Myocardial Infarction
Biography
Biography: Wen-Yih Chen
Abstract
Cardiac troponin I (cTnI) has been referred to and considered as the gold standard for diagnosing acute myocardial infarction (AMI) and monitoring the variation of this biomarker in human blood within short period is critical for early-stage diagnosis of this disease. However, precisely quantifying cTnI is still challenging, especially at its ultra-low level, due to insensitivity and long time-to-result of the current techniques. The purpose of this study is to develop a biosensor, which can quantify cTnI at ultra-low concentrations in human serum with high resolution and short time-to-result for early-stage diagnosis of AMI.Methodology and Theoretical Orientation: The silica substrates were modified with (3-aminopropyl)triethoxysilane (APTES), 1-(3-aminopropyl)silatrane (APS), and silane-polyethylene glycol (silane-PEG) to immobilize cTnI aptamer as the bio-probe. The morphology and topology of the modified surfaces were investigated by atomic force microscopy (AFM) whereas their ability to resist non-specific foulants was also examined by fibrinogen adsorption. The SiNWFET aptasensors developed from these modification methods were employed to detect cTnI at ultra-low content and small increment in human serum.