Jeremiah J. Morrissey
Siteman Cancer Center, USA
Title: Plasmonic-fluor Enhanced Minimally Invasive Microneedle Patches to Sample Interstitial Fluid for Biomarkers of Health and Disease
Biography
Biography: Jeremiah J. Morrissey
Abstract
The detection and quantification of protein biomarkers in interstitial fluid is hampered by challenges in its sampling and analysis. Here we report the use of a microneedle patch for fast in vivo sampling and on-needle quantification of target protein or low molecular weight biomarkers of health and disease in interstitial fluid. We used plasmonic fluor, an ultrabright fluorescent label, to improve the limit of detection of various interstitial fluid biomarkers by nearly 800-fold compared with conventional fluorophores, and a magnetic backing layer to implement conventional immunoassay procedures on the patch and thus improve measurement consistency. We used the microneedle patch in mice for minimally invasive evaluation of vaccine efficacy, for longitudinal monitoring of the levels of inflammatory and kidney injury biomarkers. Additionally, we illustrate efficient sampling of the calvarial periosteum, a challenging site for biomarker detection, and quantification of the matricellular protein periostin, which cannot be accurately measured from blood or other systemic biofluids. Microneedle patches for the minimally invasive collection and analysis of biomarkers in interstitial fluid may facilitate point-of-care diagnostics and longitudinal monitoring.